Coding

Part:BBa_K4769208:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


gyrA coding sequence from B. subtilis 3610


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1816
    Illegal SpeI site found at 2247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1816
    Illegal NheI site found at 2299
    Illegal SpeI site found at 2247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1816
    Illegal BglII site found at 870
    Illegal BglII site found at 1494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1816
    Illegal SpeI site found at 2247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1816
    Illegal SpeI site found at 2247
    Illegal NgoMIV site found at 517
    Illegal AgeI site found at 129
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 100
    Illegal SapI.rc site found at 2389


Design Notes

N/A


Source

B. subtilis 3610 genome

Primers for cloning: FW: atgagtgaacaaaacacaccaca (+ desired overhangs) RV: tcacacttcttcttgttcttcttcattc (+ desired overhangs)


References